Journal: bioRxiv

Article Title: DNA Double-decker Ring Scaffolded Nanodisc for Self-assembly of Membrane Protein into Lipid Bilayer

doi: 10.64898/2026.05.19.726119

Figure Lengend Snippet: ( A ) Schematic of DNA double-decker ring (DDring) assembly. A circular ssDNA minicircle (ssMC) is annealed with seven staples modified with 8 phosphorothioates (PS) each to which alkyl moieties are attached. The alkyl moieties position inside the double-decker with the feature of membrane scaffolding hydrophobic rims. The complementary staples hybridize with the ssMC to form a dsDNA minicircle (dsMC), which in turn fold it into two decks through crossovers, ultimately forming dsDNA double-decker with the estimated dimension of ∼16.7 nm × 4 nm. ( B ) SEC profile of ssMC after exonuclease treatment. SEC was run at 0.5 mL/min elution rate with the buffer composed of 200 mM Na 2 SO 4 , 5 mM MgSO 4 and 50 mM HEPES (pH 7.4) using Superose TM 6 Increase 10/300.

Article Snippet: SEC was performed by JASCO HPLC system using either Superose TM 6 Increase 10/300 GL column (Cytiva) at a flow rate of 0.5 mL/min or 3.2/300 GL column (Cytiva) at 0.04 mL/min and eluted with the buffer (10 mM HEPES (pH 7.4), 150 mM NaCl, 5 mM MgCl 2 ).

Techniques: Modification, Membrane, Scaffolding

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Figure Lengend Snippet: Proteins were mixed in an equimolar ratio, incubated on ice for 1 hr and run on a Superose 6 increase 3.2/300 column. Eluted proteins were visualized by SDS-PAGE and Coomassie staining. For the XLMS analysis shown in the pre-incubated complex was cross-linked prior to SEC, and elution fractions 12 and 13 corresponding to the non-cross-linked complex were pooled and digested for mass spectrometric analysis.

Article Snippet: Analytical SEC experiments were performed on a Superdex 200 Increase 3.2/300 or a Superose 6 Increase 3.2/300 column (GE Healthcare).

Techniques: Incubation, SDS Page, Staining

Journal: Molecular Cell

Article Title: Scc2 Is a Potent Activator of Cohesin’s ATPase that Promotes Loading by Binding Scc1 without Pds5

doi: 10.1016/j.molcel.2018.05.022

Figure Lengend Snippet:

Article Snippet: Peak fractions were analyzed using SDS-PAGE and were further purified in a Superose 6 Increase 10/300 (VWR) using HNTG as running buffer (free of EDTA/PMSF).

Techniques: Recombinant, Protease Inhibitor, Western Blot, Transfection, Software